Facility and Equipment

The IHCF is a multi-user service center located in Room 103 of the Wettaw Building, IHCF has been serving the Flagstaff community for more than 40 years, and houses multiple high powered microscopes, ancillary equipment, and histological equipment to meet the diverse research needs of University faculty, students and industry partners.

The IHCF is equipped with a scanning electron microscope, transmission electron microscope, and a confocal microscope as well as specimen preparation areas.

 

 

Electron microscopes

• Zeiss Supra 40VP field emission scanning electron microscope (SEM)
  • Backscattered electron detectors-AsB and Robinson
  • Secondary electron detectors-Everhart Thornley and InLens
  • Variable pressure analysis of non-conducting and wet specimens
  • Thermo Scientific UltraDry energy dispersive x-ray spectrometer (EDS)
  • Thermo Scientific MagnaRay wavelength dispersive x-ray spectrometer (WDS)
• JEOL JEM 1200EX-II transmission electron microscope (TEM)
• JEOL JSM-6480LV SEM
  • Oxford Inca X-Sight EDS
  • Oxford Inca Wave WDS
• Cameca SX-50 microprobe

Sample preparation equipment

• Pelco CPD-2 critical point dryer
• Emitech K750 freeze dryer
• Denton Vacuum Desk II cold sputter etch unit with carbon evaporation accessory
• Reichert-Jung Ultracut E ultramicrotome
• Leica EM UC7 ultramicrotome

Microscopes

• Leitz Arisoplan Variophot light microscope
• Zeiss Discovery. V8 stereo microscope
  • AxioCam MRm monochrome camera
  • X-Cite Series 120 Q fluorescence unit with DsRed (for RFP) Cube and GFP Cube Bandpass filters
  • Transmitted and reflected light sources available
• Zeiss AxioScope.A1 light microscope
  • AxioCam MRc5 color camera
  • DAPI, AF488, AF546, and Long Pass 420 filters
• Keyence VHX-2000 digital microscope
  • 20x-5000x high resolution
  • 3D imaging and measurement capabilities
• Leica DMIRE2 spinning disk confocal microscope
• Leica TCS SPE II confocal microscope
  • Brightfield, fluorescence, DIC, confocal scanning and polarized light capabilities
  • OKO incubation chamber for live cell experiments
  • 10x, 20x (multi-immersion), 40x (oil), 63x (oil)
  • Confocal laser lines
    • 405 nm, 488 nm, 561 nm, 635 nm
  • Fluorescence filters
    • DAPI excitation: 350/50, emission: BP 460/50, FITC excitation: BP 480/40, emission: BP 527/30, Texas Red excitation: BP 560/40, emission: BP 630/75

Histology equipment

• Thermo Scientific Excelsior AS tissue processor
• Leica EG 1150H tissue embedding station
• Leica RM2255 microtome x2
• Leica Jung Frigocut 2800N cryostat
• Leica Aperio ScanScope CS2 digital slide scanner