Facility and Equipment

The Imaging Histology Core Facility (IHCF) is a multi-user service center located in room 103 of the Wettaw building. The IHCF has been serving the Flagstaff community for more than 40 years, and houses multiple high-powered microscopes, ancillary equipment, and histological equipment to meet the diverse research needs of university faculty, students, and industry partners.

The IHCF is equipped with a scanning electron microscope, transmission electron microscope, and a confocal microscope as well as specimen preparation areas.

Electron microscopes

• Zeiss Supra 40VP field emission scanning electron microscope (SEM)
  • Backscattered electron detectors-AsB and Robinson
  • Secondary electron detectors-Everhart Thornley and InLens
  • Variable pressure analysis of non-conducting and wet specimens
  • Thermo Scientific UltraDry energy dispersive x-ray spectrometer (EDS)
  • Thermo Scientific MagnaRay wavelength dispersive x-ray spectrometer (WDS)
• JEOL JEM 1200EX-II transmission electron microscope (TEM)
• JEOL JSM-6480LV SEM
  • Oxford Inca X-Sight EDS
  • Oxford Inca Wave WDS
• Cameca SX-50 microprobe

Sample preparation equipment

• Pelco CPD-2 critical point dryer
• Emitech K750 freeze dryer
• Denton Vacuum Desk II cold sputter etch unit with carbon evaporation accessory
• Reichert-Jung Ultracut E ultramicrotome
• Leica EM UC7 ultramicrotome

Microscopes

• Leitz Arisoplan Variophot light microscope
• Zeiss Discovery. V8 stereo microscope
  • AxioCam MRm monochrome camera
  • X-Cite Series 120 Q fluorescence unit with DsRed (for RFP) Cube and GFP Cube Bandpass filters
  • Transmitted and reflected light sources available
• Zeiss AxioScope.A1 light microscope
  • AxioCam MRc5 color camera
  • DAPI, AF488, AF546, and Long Pass 420 filters
• Keyence VHX-2000 digital microscope
  • 20x-5000x high resolution
  • 3D imaging and measurement capabilities
• Leica DMIRE2 spinning disk confocal microscope
• Leica TCS SPE II confocal microscope
  • Brightfield, fluorescence, DIC, confocal scanning and polarized light capabilities
  • OKO incubation chamber for live cell experiments
  • 10x, 20x (multi-immersion), 40x (oil), 63x (oil)
  • Confocal laser lines
    • 405 nm, 488 nm, 561 nm, 635 nm
  • Fluorescence filters
    • DAPI excitation: 350/50, emission: BP 460/50, FITC excitation: BP 480/40, emission: BP 527/30, Texas Red excitation: BP 560/40, emission: BP 630/75

Histology equipment

• Thermo Scientific Excelsior AS tissue processor
• Leica EG 1150H tissue embedding station
• Leica RM2255 microtome x2
• Leica Jung Frigocut 2800N cryostat
• Leica Aperio ScanScope CS2 digital slide scanner