{"id":289,"date":"2018-10-08T23:49:55","date_gmt":"2018-10-08T23:49:55","guid":{"rendered":"https:\/\/in.nau.edu\/pmi\/?page_id=289"},"modified":"2018-11-30T15:30:26","modified_gmt":"2018-11-30T22:30:26","slug":"facilities","status":"publish","type":"page","link":"https:\/\/in.nau.edu\/pmi\/pathogen-microbiome-institute\/facilities\/","title":{"rendered":"Facilities"},"content":{"rendered":"<h1>Facilities<\/h1>\n<hr \/>\n<h2>Ancient DNA laboratory<\/h2>\n<figure id=\"attachment_387\" aria-describedby=\"caption-attachment-387\" style=\"width: 465px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-tall-banner-image-sm wp-image-387\" src=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/PetrifiedPoop-465x186.jpg\" alt=\"PetrifiedPoop\" width=\"465\" height=\"186\" \/><figcaption id=\"caption-attachment-387\" class=\"wp-caption-text\">PMI UG Research Assistants examine a subfossil dung sample.<\/figcaption><\/figure>\n<p>The <a href=\"https:\/\/in.nau.edu\/pmi\/pathogen-microbiome-institute\/ancient-dna-lab\/\">Ancient DNA Lab<\/a> provides support for researchers working with ancient, historical, forensic, or other sensitive (low DNA quantity\/quality) genetic samples.<\/p>\n<hr \/>\n<h2>Biosafety Level 2 (BSL-2) laboratory<\/h2>\n<figure id=\"attachment_392\" aria-describedby=\"caption-attachment-392\" style=\"width: 465px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-tall-banner-image-sm wp-image-392\" src=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL2_BSC-465x186.jpg\" alt=\"BSL2_BSC\" width=\"465\" height=\"186\" srcset=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL2_BSC-465x186.jpg 465w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL2_BSC-960x384.jpg 960w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL2_BSC-1440x576.jpg 1440w\" sizes=\"auto, (max-width: 465px) 100vw, 465px\" \/><figcaption id=\"caption-attachment-392\" class=\"wp-caption-text\">A PMI staff and UG work in one of several BSCs within PMI&#8217;s BSL-2.<\/figcaption><\/figure>\n<p><a href=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL2_biosafety.png\">BSL-2<\/a> is suitable for work involving agents that pose moderate hazards to personnel and the environment such as <em><a href=\"https:\/\/www.cdc.gov\/ecoli\/\">Escherichia coli<\/a><\/em> and <a href=\"https:\/\/www.cdc.gov\/mrsa\/\">MRSA<\/a>. Laboratory personnel have specific training in handling pathogenic agents, access is restricted and all procedures are conducted in BSCs or other physical containment equipment.<\/p>\n<hr \/>\n<h2>Biosafety Level 3 (BSL-3) high-containment laboratory<\/h2>\n<figure id=\"attachment_393\" aria-describedby=\"caption-attachment-393\" style=\"width: 472px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-393 \" src=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL3_BSC-600x337.jpg\" alt=\"BSL3_BSC\" width=\"472\" height=\"265\" srcset=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL3_BSC-600x337.jpg 600w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL3_BSC-300x168.jpg 300w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL3_BSC-768x431.jpg 768w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL3_BSC-1024x575.jpg 1024w\" sizes=\"auto, (max-width: 472px) 100vw, 472px\" \/><figcaption id=\"caption-attachment-393\" class=\"wp-caption-text\">A PMI BSL-3 technician wearing a Powered Air Purifying Respirator (PAPR) works in a BSC.<\/figcaption><\/figure>\n<p><a href=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/BSL3_biosafety.png\">BSL-3<\/a> is applicable to research\u00a0facilities where work is performed with biological agents that have the potential to pose a severe threat to public health and safety. Laboratory personnel receive specific training in handling potentially lethal agents. All procedures must be conducted within BSCs. The CDC regulates and inspects BSL-3 laboratories that conduct research on <a href=\"https:\/\/www.selectagents.gov\/\">Select Agents<\/a>.<\/p>\n<hr \/>\n<h2>Caporaso Bioinformatics Lab<\/h2>\n<figure id=\"attachment_405\" aria-describedby=\"caption-attachment-405\" style=\"width: 476px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-405 \" src=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/Capo_cropped-300x202.jpg\" alt=\"Concourse\" width=\"476\" height=\"321\" srcset=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/Capo_cropped-300x202.jpg 300w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/Capo_cropped-768x516.jpg 768w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/Capo_cropped-1024x689.jpg 1024w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/Capo_cropped-600x404.jpg 600w\" sizes=\"auto, (max-width: 476px) 100vw, 476px\" \/><figcaption id=\"caption-attachment-405\" class=\"wp-caption-text\">Concourse monitoring and debugging updates to Qiime 2.<\/figcaption><\/figure>\n<p>The <a href=\"http:\/\/caporasolab.us\/\">Caporaso Bioinformatics Lab<\/a> is an applied bioinformatics lab focused on microbiome research, software development, and bioinformatics education.<\/p>\n<hr \/>\n<h2>Genetic Analysis Instrument Lab<\/h2>\n<figure id=\"attachment_414\" aria-describedby=\"caption-attachment-414\" style=\"width: 466px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-414\" src=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/1012181112b_Film1-300x168.jpg\" alt=\"Genetic Analysis Instrument Lab\" width=\"466\" height=\"261\" srcset=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/1012181112b_Film1-300x168.jpg 300w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/1012181112b_Film1-768x431.jpg 768w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/1012181112b_Film1-1024x575.jpg 1024w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/1012181112b_Film1-600x337.jpg 600w\" sizes=\"auto, (max-width: 466px) 100vw, 466px\" \/><figcaption id=\"caption-attachment-414\" class=\"wp-caption-text\">QuantStudio 7 Flex, QuantStudio 12K Flex and 7500 Fast: Real-Time PCR Systems.<\/figcaption><\/figure>\n<p>Real-Time PCR,\u00a0developed in 1992, is used to exponentially\u00a0amplify\u00a0a single copy of a segment of\u00a0DNA. The Real-Time instrument\u00a0allows for the detection of the anticipated DNA target region during the early phases of the reaction.<\/p>\n<hr \/>\n<h2>PCR Amplification Lab<\/h2>\n<figure id=\"attachment_408\" aria-describedby=\"caption-attachment-408\" style=\"width: 468px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-408\" src=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/AmpliconLab-300x168.jpg\" alt=\"PCR Thermal Cyclers\" width=\"468\" height=\"262\" srcset=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/AmpliconLab-300x168.jpg 300w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/AmpliconLab-768x431.jpg 768w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/AmpliconLab-1024x575.jpg 1024w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/AmpliconLab-600x337.jpg 600w\" sizes=\"auto, (max-width: 468px) 100vw, 468px\" \/><figcaption id=\"caption-attachment-408\" class=\"wp-caption-text\">Thermal Cyclers in PMI&#8217;s Traditional PCR Amplification Lab.<\/figcaption><\/figure>\n<p>Traditional PCR was developed in 1983. It&#8217;s\u00a0used to exponentially\u00a0amplify\u00a0a single copy of a segment of\u00a0DNA, then once the reaction is complete uses agarose gel electrophoresis to check whether the reaction successfully generated the anticipated DNA target region.<\/p>\n<hr \/>\n<h2>TGen North Pathogen and Microbiome Division<\/h2>\n<figure id=\"attachment_402\" aria-describedby=\"caption-attachment-402\" style=\"width: 441px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-402\" src=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/tgen-north-lrg-300x142.jpg\" alt=\"TGen North logo\" width=\"441\" height=\"209\" srcset=\"https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/tgen-north-lrg-300x142.jpg 300w, https:\/\/in.nau.edu\/wp-content\/uploads\/sites\/216\/2018\/10\/tgen-north-lrg.jpg 371w\" sizes=\"auto, (max-width: 441px) 100vw, 441px\" \/><figcaption id=\"caption-attachment-402\" class=\"wp-caption-text\">TGen North adds translational genomics capabilities to PMI&#8217;s research.<\/figcaption><\/figure>\n<p><a href=\"https:\/\/www.tgen.org\/patients\/pathogen-microbiome\/\">TGen North<\/a> focuses on diagnostic, analytic, forensic, ecologic and epidemiologic research of microbes important to medicine, public health and biodefense.<\/p>\n<hr \/>\n","protected":false},"excerpt":{"rendered":"<p>Facilities Ancient DNA laboratory The Ancient DNA Lab provides support for researchers working with ancient, historical, forensic, or other sensitive (low DNA quantity\/quality) genetic samples. Biosafety Level 2 (BSL-2) laboratory BSL-2 is suitable for work involving agents that pose moderate hazards to personnel and the environment such as Escherichia coli and MRSA. Laboratory personnel have [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":97,"parent":56,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_relevanssi_hide_post":"","_relevanssi_hide_content":"","_relevanssi_pin_for_all":"","_relevanssi_pin_keywords":"","_relevanssi_unpin_keywords":"","_relevanssi_related_keywords":"","_relevanssi_related_include_ids":"","_relevanssi_related_exclude_ids":"","_relevanssi_related_no_append":"","_relevanssi_related_not_related":"","_relevanssi_related_posts":"","_relevanssi_noindex_reason":"","ring_central_script_selection":"","footnotes":""},"class_list":["post-289","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/in.nau.edu\/pmi\/wp-json\/wp\/v2\/pages\/289","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/in.nau.edu\/pmi\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/in.nau.edu\/pmi\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/in.nau.edu\/pmi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/in.nau.edu\/pmi\/wp-json\/wp\/v2\/comments?post=289"}],"version-history":[{"count":51,"href":"https:\/\/in.nau.edu\/pmi\/wp-json\/wp\/v2\/pages\/289\/revisions"}],"predecessor-version":[{"id":705,"href":"https:\/\/in.nau.edu\/pmi\/wp-json\/wp\/v2\/pages\/289\/revisions\/705"}],"up":[{"embeddable":true,"href":"https:\/\/in.nau.edu\/pmi\/wp-json\/wp\/v2\/pages\/56"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/in.nau.edu\/pmi\/wp-json\/wp\/v2\/media\/97"}],"wp:attachment":[{"href":"https:\/\/in.nau.edu\/pmi\/wp-json\/wp\/v2\/media?parent=289"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}