{"id":62890,"date":"2021-05-28T11:47:23","date_gmt":"2021-05-28T18:47:23","guid":{"rendered":"https:\/\/in.nau.edu\/news\/?p=62890"},"modified":"2021-05-28T11:47:46","modified_gmt":"2021-05-28T18:47:46","slug":"rabbit-fever-virus","status":"publish","type":"post","link":"https:\/\/in.nau.edu\/news\/rabbit-fever-virus\/","title":{"rendered":"NAU scientists: Bacterium causing deadly rabbit fever remains virulent for months in cold water \u00a0"},"content":{"rendered":"

Although it is not spread through human contact, Francisella tularensis<\/em>\u00a0is one of the most infectious pathogenic bacteria known to science\u2014so virulent, in fact, that it is considered a serious potential bioterrorist threat. It is thought that humans can contract respiratory tularemia, or rabbit fever\u2014a rare and deadly disease\u2014by inhaling as few as 10 airborne organisms.<\/p>\n

Northern Arizona University professor\u00a0David Wagner<\/strong>, director of the Pathogen and Microbiome Institute\u2019s (PMI) Biodefense and Disease Ecology Center, began a three-year project in 2018 to better understand the life cycle and behavior of\u00a0F. tularensis<\/em>, funded through a $2.25 million grant from the U.S. Defense Threat Reduction Agency (DTRA).<\/p>\n

One of the most puzzling behaviors of the pathogen is its ability to remain dormant, possibly in what is called a \u201cviable but nonculturable\u201d state\u2014which means the bacteria is alive, but cannot be grown in the laboratory. That makes it much more difficult to study, because scientists can typically only study bacteria that can be cultured. Wagner\u2019s goal was to study the bacterium to determine the environmental and genetic factors that contribute to the pathogen\u2019s ability to apparently remain dormant for months at a time\u2014a phenomenon that has remained mostly a mystery despite more than 100 years of research.<\/p>\n

Now, Wagner and his collaborators have published their findings, \u201cLong-Term Survival of Virulent Tularemia Pathogens outside a Host in Conditions That Mimic Natural Aquatic Environments,\u201d in the journal Applied and Environmental Microbiology<\/em><\/a>. In the paper, the team shows how they were able to prove, by replicating environmental conditions in the lab, including low temperatures and low-nutrient water, that the bacterium can persist for months in cold water without any nutrients and remain fully virulent. Their results provide a plausible explanation for how it can overwinter in the environment outside of a host.<\/p>\n

\u201cWe are making some very interesting discoveries in this project,\u201d Wagner said. \u201cThe main finding is that Francisella tularensis<\/em> can persist in a dormant state for more than six months in cold water without any nutrients. This means it has the ability to persist in the environment outside of a mammalian host or arthropod vector. This was unexpected because many other bacteria that persist like that long-term in the environment form spores when they are outside of a host, such as Bacillus anthracis<\/em>, the bacterium that causes anthrax forms spores, but F. tularensis<\/em> doesn\u2019t do that. Others, like Yersinia pestis<\/em>\u2014the bacterium that causes plague\u2014are always either in a mammalian host or a flea vector. F. tularensis<\/em> has the ability to persist long-term in the environment long-term outside of a host without forming spores while remaining fully virulent.\u201d<\/p>\n

\u201cThese study results have completely changed our perspective on the ecology of this bacterium. We now understand that mammals are likely just a small (but still important) aspect of its survival strategy. We now think that it spends most of its time in the environment outside of a host and only periodically causes disease in mammals. But those disease events in mammals are still very important as they serve to amplify the amount of F. tularensis<\/em> that is deposited back in the environment.\u201d<\/p>\n

Working with co-principal investigator\u00a0Jason Sahl<\/strong>, associate professor and assistant director of PMI, and with PMI senior research scientists Dawn Birdsell<\/strong> and Joe Busch<\/strong>, Wagner conducted the study along with colleagues at two of the team\u2019s long-term collaborating institutions in Sweden: The Swedish Defence Research Agency and Ume\u00e5\u00a0University.<\/p>\n

Along with their Swedish collaborators, Wagner and his team are known worldwide for their work developing the phylogeny, or global family tree, of\u00a0F. tularensis<\/em>\u00a0and its phylogeography\u2014mapping where different groups of the species are found throughout the world and understanding the species\u2019 genetic diversity.<\/p>\n

\u201cAs we continue with the DTRA research grant, we are now investigating the genes and proteins that regulate the ability of F. tularensis<\/em> to persist in the environment outside of mammals and hosts. This work involves a number of current and recently graduated undergraduate students at NAU: former student Kathleen<\/strong> Soria<\/strong>, current students Natalie Hart<\/strong> and Rebecca Ballard<\/strong> and current student and Flinn Scholar Kailee Savage<\/strong>.<\/p>\n

Although the bacteria are naturally occurring throughout the northern hemisphere, including Arizona, the number of reported cases in the U.S. is small, with only 230 cases in 2016, three of which occurred in Arizona. Humans can be infected through insect bites; by drinking contaminated water, which happens in developing countries such as Turkey; by handling infected animals; and by breathing aerosolized particles containing the bacteria. Humans cannot transmit the disease to other humans. There is no vaccine to prevent the disease, which is treated with antibiotics. Wagner\u2019s team recently completed a related project funded by the DTRA studying the pathogen\u2019s antibiotic resistance.<\/p>\n

\"Northern<\/a><\/p>\n

 <\/p>\n

Kerry Bennett | Office of the Vice President for Research<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Although it is not spread through human contact, Francisella tularensis\u00a0is one of the most infectious pathogenic bacteria known to science\u2014so virulent, in fact, that it is considered a serious potential bioterrorist threat. It is thought that humans can contract respiratory tularemia, or rabbit fever\u2014a rare and deadly disease\u2014by inhaling as few as 10 airborne organisms.…<\/a><\/p>\n","protected":false},"author":59,"featured_media":52459,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[11],"tags":[],"class_list":["post-62890","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research-academics"],"acf":[],"_links":{"self":[{"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/posts\/62890","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/users\/59"}],"replies":[{"embeddable":true,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/comments?post=62890"}],"version-history":[{"count":0,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/posts\/62890\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/media\/52459"}],"wp:attachment":[{"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/media?parent=62890"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/categories?post=62890"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/tags?post=62890"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}