{"id":60659,"date":"2020-09-10T14:03:52","date_gmt":"2020-09-10T21:03:52","guid":{"rendered":"http:\/\/in.nau.edu\/news\/?p=60659"},"modified":"2020-09-10T14:03:52","modified_gmt":"2020-09-10T21:03:52","slug":"johnson-doe-grant","status":"publish","type":"post","link":"https:\/\/in.nau.edu\/news\/johnson-doe-grant\/","title":{"rendered":"NAU researcher receives $1.3 million DOE grant to study how fungus may help grow sorghum for biofuels"},"content":{"rendered":"

For years, researchers have been trying to harness the crop-growing benefits of the symbiotic relationship between plants and \"Nancy<\/a>fungi. Arbuscular mycorrhizal fungi (AMF), for example, can deliver water and nutrients to the roots of plants like sorghum, a grain used to produce biofuels. The U.S. Department of Energy (DOE) is particularly interested in producing sorghum more efficiently to reduce the need for irrigation and fertilizer and recently awarded Regents\u2019 Professor Nancy Johnson<\/strong> of Northern Arizona University\u2019s School of Earth and Sustainability<\/u> a $1.3 million grant to study the beneficial associations between biofuel sorghum and AMF.<\/p>\n

Johnson\u2019s research is part of a five-year collaboration with the University of Georgia, funded by a total of $11.7 million from the DOE, which will involve genetic sequencing and artificial intelligence. A series of field and greenhouse experiments will examine the genetic and environmental factors that cause the variable performance of mycorrhizal symbioses in field conditions.<\/p>\n

\u201cDespite decades of efforts, successful use of AMF as biofertilizers has been elusive, largely because mycorrhizae are complex systems and their effects are context dependent,\u201d Johnson said. \u201cEnvironmental conditions, and especially soil fertility and water availability, influence the degree to which AMF can improve sorghum production. Also, different genetic lines of sorghum vary in mycorrhizal responsiveness, and complex communities of AMF and other soil microbes differ in their effects on the nutrient and water relations of their host plants.\u201d<\/p>\n

Scientists will be conducting shotgun (metagenomic) sequencing to understand what microscopic communities exist in samples of sorghum roots.<\/p>\n

\u201cShotgun sequencing reveals all the genetic sequences in samples,\u201d Johnson said. \u201cUntil recently, there was too much information, but now with advanced computing and bioinformatics, you can figure out what a lot of the organisms are. We\u2019re recognizing there\u2019s kind of a dance between the plant roots and AMF, and to a certain extent, the plant can select which fungi it associates with to form mycorrhizal symbioses. This research will help us learn more about the process by which plants control their microbiome under different environmental conditions.\u201d<\/p>\n

The researchers will be growing more than 350 different genotypes of sorghum at agricultural experiment stations in Maricopa County, Arizona, and near Athens, Georgia, to examine how sorghum genetics are interacting with AMF and other organisms in their microbiome.<\/p>\n

\u201cWe\u2019ll characterize which microbes are active in their roots by measuring RNA as well as the DNA. Once we see what\u2019s going on in agricultural fields, we\u2019ll make single-spore cultures of AMF and grow them in controlled experiments,\u201d Johnson said.<\/p>\n

Systems models will be constructed that link sorghum performance with the communities of AMF and other microbes in the field experiments, and these models will be tested in the NAU Research Greenhouse<\/u> complex.<\/p>\n

To complement the genomic and transcriptomic work, the scientists will apply novel imaging tools to visualize the interactions between sorghum roots and AMF. Johnson will use microscopy in her Soil<\/u><\/p>\n

Ecology Lab<\/u> at NAU to identify fungal allocation patterns, which will add a spatial context to the research.<\/p>\n

\u201cWe\u2019ll stain the AMF in the roots and examine them under a light microscope. We\u2019ll take pictures and use artificial intelligence to teach the computer how to recognize and quantify fungal structures in thousands of root samples.\u201d<\/p>\n

Using innovative convolutional neural network software, the computer will be able to identify AMF and also non-mycorrhizal fungi.<\/p>\n

\u201cThe Department of Energy wants to be able to produce biofuels more sustainably by helping growers of sorghum select the genotypes that grow best in their environment and not need additional fertilizer. These studies will help advance basic understanding of the genetic mechanisms by which plants control their associations with potentially beneficial root-associated microbes,\u201d Johnson said. \u201cA better understanding of natural communities of AMF in soils and the mechanisms that influence mycorrhizal functioning in field conditions is necessary before effective methods can be developed to manage AMF to increase sustainable biofuel sorghum production.\u201d<\/p>\n

Johnson awarded Bullard Fellowship to study microbiome evolution at Harvard<\/strong><\/h3>\n

Johnson is on sabbatical, working with several researchers at Harvard University to study the evolution of microbiomes through the Bullard Fellowship she was recently awarded. The DOE sorghum project complements her sabbatical research by providing an empirical study system to test evolutionary theories about the assembly of microbiomes in plants.<\/p>\n

\"Northern<\/a><\/p>\n

 <\/p>\n

Kerry Bennett | Office of the Vice President for Research<\/p>\n","protected":false},"excerpt":{"rendered":"

For years, researchers have been trying to harness the crop-growing benefits of the symbiotic relationship between plants and fungi. Arbuscular mycorrhizal fungi (AMF), for example, can deliver water and nutrients to the roots of plants like sorghum, a grain used to produce biofuels. The U.S. Department of Energy (DOE) is particularly interested in producing sorghum…<\/a><\/p>\n","protected":false},"author":59,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[11],"tags":[],"class_list":["post-60659","post","type-post","status-publish","format-standard","hentry","category-research-academics"],"acf":[],"_links":{"self":[{"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/posts\/60659","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/users\/59"}],"replies":[{"embeddable":true,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/comments?post=60659"}],"version-history":[{"count":0,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/posts\/60659\/revisions"}],"wp:attachment":[{"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/media?parent=60659"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/categories?post=60659"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/in.nau.edu\/news\/wp-json\/wp\/v2\/tags?post=60659"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}